| Title | The importance of selecting the right internal control gene to study the effects of antenatal glucocorticoid administration in human placenta. |
| Publication Type | Journal Article |
| Year of Publication | 2016 |
| Authors | Gütling, H, Bionaz, M, Sloboda, DM, Ehrlich, L, Braun, F, Gramzow, AK, Henrich, W, Plagemann, A, Braun, T |
| Journal | Placenta |
| Volume | 44 |
| Pagination | 19-22 |
| Date Published | 2016 Aug |
| ISSN | 1532-3102 |
| Abstract | RT-qPCR requires a suitable set of internal control genes (ICGs) for an accurate normalization. The usefulness of 7 previously published ICGs in the human placenta was analyzed according to the effects of betamethasone treatment, sex and fetal age. Raw RT-qPCR data of the ICGs were evaluated using published algorithms. The algorithms revealed that a reliable normalization was achieved using the geometrical mean of PPIA, RPL19, HMBS and SDHA. The use of a different subset ICGs out of the 7 investigated, although not statistically affected by the conditions, biased the results, as demonstrated through changes in expression of glucocorticoid receptor (NR3C1) mRNA as a target gene. |
| DOI | 10.1016/j.placenta.2016.05.011 |
| Alternate Journal | Placenta |
| PubMed ID | 27452434 |
