The importance of selecting the right internal control gene to study the effects of antenatal glucocorticoid administration in human placenta.

TitleThe importance of selecting the right internal control gene to study the effects of antenatal glucocorticoid administration in human placenta.
Publication TypeJournal Article
Year of Publication2016
AuthorsG├╝tling, H, Bionaz, M, Sloboda, DM, Ehrlich, L, Braun, F, Gramzow, AK, Henrich, W, Plagemann, A, Braun, T
JournalPlacenta
Volume44
Pagination19-22
Date Published2016 Aug
ISSN1532-3102
Abstract

RT-qPCR requires a suitable set of internal control genes (ICGs) for an accurate normalization. The usefulness of 7 previously published ICGs in the human placenta was analyzed according to the effects of betamethasone treatment, sex and fetal age. Raw RT-qPCR data of the ICGs were evaluated using published algorithms. The algorithms revealed that a reliable normalization was achieved using the geometrical mean of PPIA, RPL19, HMBS and SDHA. The use of a different subset ICGs out of the 7 investigated, although not statistically affected by the conditions, biased the results, as demonstrated through changes in expression of glucocorticoid receptor (NR3C1) mRNA as a target gene.

DOI10.1016/j.placenta.2016.05.011
Alternate JournalPlacenta
PubMed ID27452434