The influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome.

TitleThe influences of parental diet and vitamin E intake on the embryonic zebrafish transcriptome.
Publication TypeJournal Article
Year of Publication2014
AuthorsMiller, GW, Truong, L, Barton, CL, Labut, EM, Lebold, KM, Traber, MG, Tanguay, RL
JournalComp Biochem Physiol Part D Genomics Proteomics
Date Published2014 Jun
KeywordsAnimals, Diet, Embryo, Nonmammalian, Female, Gene Expression Regulation, Developmental, Male, Transcriptome, Vitamin E, Zebrafish

The composition of the typical commercial diet fed to zebrafish can dramatically vary. By utilizing defined diets we sought to answer two questions: 1) How does the embryonic zebrafish transcriptome change when the parental adults are fed a commercial lab diet compared with a sufficient, defined diet (E+)? 2) Does a vitamin E-deficient parental diet (E-) further change the embryonic transcriptome? We conducted a global gene expression study using embryos from zebrafish fed a commercial (Lab), an E+ or an E- diet. To capture differentially expressed transcripts prior to onset of overt malformations observed in E- embryos at 48h post-fertilization (hpf), embryos were collected from each group at 36hpf. Lab embryos differentially expressed (p<0.01) 946 transcripts compared with the E+ embryos, and 2656 transcripts compared with the E- embryos. The differences in protein, fat and micronutrient intakes in zebrafish fed the Lab compared with the E+ diet demonstrate that despite overt morphologic consistency, significant differences in gene expression occurred. Moreover, functional analysis of the significant transcripts in the E- embryos suggested perturbed energy metabolism, leading to overt malformations and mortality. Thus, these findings demonstrate that parental zebrafish diet has a direct impact on the embryonic transcriptome.

Alternate JournalComp. Biochem. Physiol. Part D Genomics Proteomics
PubMed ID24657723
PubMed Central IDPMC4037372
Grant ListP30 ES000210 / ES / NIEHS NIH HHS / United States
R01 HD062109 / HD / NICHD NIH HHS / United States
RC4 ES019764 / ES / NIEHS NIH HHS / United States